BIO 240(E) - Cell Culture and Protein Purification

Credit(s): 4
MassTransfer Block Code: Natural & Physical Laboratory Science
This course is designed to provide students with an introduction to the techniques involved in the production of a recombinant protein, including an in-depth discussion of both cell culture (upstream processing) and protein purification (downstream processing). Students in this course will learn how to successfully preserve and maintain different cell lines, and use cells to express a recombinant protein. Students will be engaged in the techniques involved in purifying the recombinant protein, including column chromatography, dialysis, and diafiltration. Validation of the cell line and protein expressed will also be covered.

Prerequisite(s): BIO 106, BIO 229, or BIO 243 with a grade of C or better

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